#1 - Helmchen Lab, Brain Research Institute, University of Zurich, Switzerland
The oldest mesoSPIM saw first light in October 2015 (as a Version 0). The most recent upgrade in May 2018 brought it up to Version 4. The microscope is accessible within collaborations with the Helmchen lab.
#3 - Advanced Lightsheet Imaging Center, Wyss Center, Geneva, Switzerland
The first mesoSPIM outside of Zurich saw first light as a Version 4 in March 2018. It is open to users from both academia and industry. The facility also contains a CLARITY-optimized light-sheet microscope (COLM) for high-resolution imaging.
#4 - Center for Microscopy and Image Analysis, University of Zurich, Switzerland
mesoSPIM #4 was set up in May 2018 by Fabian Voigt, and upgraded in 2023 by Nikita Vladimirov. It is open to users of the Center for Microscopy and Image Analysis (UZH labs, partner institutes and private companies). It features 5 plan apochromat objectives (2x - 20x) and a motorized revolver from Mitutoyo, along with large-sensor camera Hamamatsu Orca Lightning.
#5 - Advanced Microscopy Facility, Sainsbury Wellcome Centre for Neural Circuits and Behaviour, London, UK
The fifth mesoSPIM (and the first outside of Switzerland) became operational in December 2018.
#6 - Aguzzi Lab, Institute of Neuropathology, University Hospital Zurich, Switzerland
The first mesoSPIM V5 was set up in early August 2019. Together with mesoSPIM #2, it forms a high-throughput imaging platform.
#9 - Ertürk lab at the Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Germany
The instrument was built by Tzu-Lun Wang, Mihail Todorov and Fabian F. Voigt and took its first images on November 8th, 2019.
#15 - Cardiac Imaging Laboratory at European Laboratory of Nonlinear Spectroscopy (LENS), Sesto Fiorentino, Italy
The first mesoSPIM in Italy was set up by Leonardo Sacconi in the summer of 2021. The microscope is used for reconstructing cleared whole mouse hearts. The detection arm uses large-sensor back-illuminated sCMOS camera with a high-resolution 2X telecentric lens.
#16 - National Facility for Translational Medicine, Shanghai, China
The first mesoSPIM in China was set up in the group of Prof. Chunhai Fan as a apart of Biological Perspective project. The mesoSPIM was set up in October 2022 and its application is focused on brain imaging and studying intact human tissue. The imaging samples are mouse brains and other model organisms. Contact: Dr. Zhaoshuai Gao.
#19 - MDIBL imaging facility (MDIBL-LMF), Bar Harbor, Maine, USA
The mesoSPIM at the MDIBL is the 5th in the USA and it was set up by the Murawala Lab with the help of Dr. Fabian Voigt in December 2022. Its access to the internal and external users is provided via the MDIBL imaging facility (MDIBL-LMF). The mesoSPIM will be used to study developmental and tissue regeneration studies in axolotl and zebrafish, aging studies in zebrafish and African turquoise killifish, and to address brain connectome questions in the mouse.
#20 - Center for Biologic Imaging, University of Pittsburgh, PA, USA
The mesoSPIM at the CBI is the 6th in the USA. It was set up by Profs. Simon Watkins and Alan Watson and Dr. Callen Wallace in February 2023 for imaging bladder, brain, spinal cord, lung and other cleared tissues. This setup was built using Nikon AZ100 zoom body, a creative variation of the original design.
#21, Benchtop mesoSPIM #1 - Helmchen Lab, Brain Research Institute, University of Zurich, Switzerland
The first Benchtop mesoSPIM, started in Helmchen Lab by Fabian Voigt in 2019 and brought to maturity by Nikita Vladimirov in 2021-2023.
#22, (Half-)Benchtop #2 at Microscopy Innovation Lab, University of Texas Southwestern Medical Center, Dallas, TX, USA
A variation of Benchtop with only one exitation arm, and the Dean lab’s second mesoSPIM. This system was set up in 2023 by Melissa Glidewell and Dr. Kevin Dean to image cleared mouse tissues and expansion microscopy samples.
#23, Benchtop #3 - Center for Biologic Imaging, University of Pittsburgh, PA, USA
A variation of Benchtop with Nikon AZ100 detection objectives, custom immersion cuvette mount, and several other minor tweaks so it matches their other system. Second mesoSPIM at CBI, built in September 2023 to expand the facility’s imaging capacity of zebrafish, rat knees, mouse brains, lungs and other biological samples.