mesoSPIM Symposium: October 13-15, 2025, Zurich, Switzerland
With nearly 150 on-site participants and 12 sponsors, the 10-year Anniversary mesoSPIM Symposium became a great platform for scientific exchange and networking in the field of light-sheet microscopy, with many new collaborations originated from it.
Booklet
Download the symposium booklet (PDF)
Program
Day 1. Monday, October 13th
| Time | Talk |
|---|---|
| 8:15-9:00 | Registration and coffee |
| Session 1 | Developers. Chair: Urs Ziegler |
| 9:00-9:10 | Fritjof Helmchen (University of Zurich, Switzerland). Welcome note. |
| 9:10-9:40 | Fabian Voigt (Harvard University, MA, USA). Expanding the bag of optical tricks for neuroscience. |
| 9:40-10:00 | Nikita Vladimirov (University of Zurich, Switzerland). The 10 years of mesoSPIM: lessons, updates and outlook. |
| 10:00-10:40 | (keynote, online) Simon C. Watkins (University of Pittsburgh, PA, USA). Pushing the mesoSPIM to submicron resolution with chemistry and computing. |
| 10:40-11:00 | Coffee break |
| Session 2 | Tissue clearing. Chair: Esther Stoeckli |
| 11:00-11:30 | (online) Anna Maria Reuss (University Hospital Zurich, Switzerland). aDISCO - A Broadly Applicable Method for 3D Microscopy of Archival Paraffin-Embedded Human Tissues. |
| 11:30-12:00 | (online) Hei Ming Lai (The Chinese University of Hong Kong). Customizing mesoSPIM-based workflows for clinical 3D tissue diagnostics. |
| 12:00-12:20 | Christophe Lamy (University of Geneva, Switzerland). Human anatomy at the age of optical dissection. |
| 12:20-12:30 | Martina Schättin Award |
| 12:30-13:00 | Lunch |
| 13:00-13:50 | Poster session, industry demos |
| Session 3 | Industrial solutions to imaging. Chair: Fabian Voigt |
| 13:50-14:10 | Ando Zehrer (Teledyne Photometrics, Germany). The Iris15: Teledyne Photometrics contribution to the MesoSPIM initiative. |
| 14:10-14:30 | Jon Daniels (Applied Scientific Instrumentation, USA). Modular Microscope and Automation Solutions. |
| 14:30-14:50 | Ralf Kruse (Mitutoyo Europe). TAGLENS – Ultrafast Focus Shift by Sound. |
| 14:50-15:10 | Otmane Buchareb (INSCOPER, France). Imaging software solution for automated and smart microscopy. |
| 15:10-15:30 | Nicolaas van der Voort (SVI Huygens, Netherlands). Powerful mesoSPIM image processing in the Huygens Software: a journey from Deconvolution, Fusion & Stitching to image visualization. |
| 15:30-16:00 | Group photo followed by a coffee break |
| Session 4 | Microscopy methods. Chair: Nikita Vladimirov |
| 16:00-16:30 | Kevin Dean (University of Texas Southwestern, TX, USA). Navigate, MCT-ASLM, and Altair-ASLM: Intelligent, Multiscale, Easy-to-Assemble Light-Sheet Platforms. |
| 16:30-17:00 | Prayag Murawala (MDI Biological Laboratory, ME, USA). Enhanced mesoSPIM for high-resolution mapping of brainstem locomotor circuits. |
| 17:00-17:20 | Jenna Hanmer (University of Nottingham, UK). Whole-brain imaging in rodents using MRI and light-sheet microscopy: A cross-scale, multi-modal approach. |
| 17:20-18:00 | (keynote, online) Adam Glaser (Allen Institute, WA, USA). New technologies for mapping centimeter scale tissues with nanoscale resolution. |
| 18:00-19:30 | Apero and get-together |
Day 2. Tuesday, October 14th
| Time | Talk |
|---|---|
| 8:30 | Welcome coffee |
| Session 5 | Big data analysis and AI. Chair: Joana Delgado Martins |
| 9:00-9:30 | Stephan Preibisch (HHMI Janelia Research Center, VA, USA). BigStitcher-Spark: Software for peta-scale alignment of light sheet microscopy acquisitions. |
| 9:30-10:00 | Alan Watson (University of Pittsburgh, PA, USA). Automating MesoSPIM Data Postprocessing. |
| 10:00-10:20 | Dimokratis Karamanlis (University of Geneva, Switzerland). LightSuite: A high-throughput pipeline for localizing experience in the cleared mouse brain. |
| 10:20-11:00 | Coffee break |
| Session 6 | Data analysis solutions. Chair: Csaba Földy |
| 11:00-11:20 | Michael Mahlert (Oxford Instruments Imaris). Interactive 3D and 4D image visualization and high throughput analysis with Imaris. |
| 11:20-11:40 | Michael Morehead (SyGlass). syGlass: Visualization, Annotation, and Communication of Very Large Image Volumes in Virtual Reality. |
| 11:40-12:00 | Tamara Manuelian (ZEISS Research Microscopy Solutions). ZEISS Beyond Imaging: How to manage, explore & analyze your imagery with ZEISS Software Solutions. |
| 12:00-12:20 | Joel Lüthi (University of Zurich, Switzerland). Fractal: An open-source framework for reproducible bioimage analysis at scale using OME-Zarrs. |
| 12:20-13:00 | Lunch |
| 13:00-13:50 | Poster session, industry demos |
| Session 7 | Applications. Chair: Fritjof Helmchen |
| 13:50-14:20 | Esther Stoeckli (University of Zurich, Switzerland). mesoSPIM facilitates the analysis of neural circuit formation. |
| 14:20-14:50 | Laura Baudis (University of Zurich, Switzerland). Probing dark matter and neutrinos with light sheet microscopy. |
| 14:50-15:20 | Christelle Langevin (INRAE, University of Paris-Saclay, France). Advancing animal health with tissue clearing and deep tissue imaging. |
| 15:20-15:50 | Coffee break |
| Session 8 | Applications. Chair: Martin Müller |
| 15:50-16:20 | Laura Batti (Wyss Geneva, Switzerland). Light-Sheet Microscopy: empowering advancement in neuroscience and medicine. |
| 16:20-16:40 | Akanksha Jain (ETH Zürich, Switzerland). Unveiling the choreography of human brain development: Longterm lightsheet imaging unveils morphodynamics in human brain organoids. |
| 16:40-17:00 | Melvin Alappat (University of Zürich, Switzerland). Clearly sweet and painful: Investigating the effect of western diet on myelin homeostasis. |
| 17:00-17:30 | Thomas Naert (Ghent University, Belgium). Pythia: AI-predictable CRISPR-Cas9 editing meets mesoSPIM for visualizing precise genomic integrations and edits. |
| 17:30-17:40 | Nikita Vladimirov. Concluding remarks |
| 17:40-19:00 | Apero and farewell |
Gallery
